Oral Biosciences & Medicine
Oral Biosci Med 2 (2005), No. 1     4. July 2005
Oral Biosci Med 2 (2005), No. 1  (04.07.2005)

Page 15-19


Periodontal Ligament Fibroblast Interleukin-6 Release after Exposure to Nicotine
Olson, Byron L./Warner, Ned A./Gregory, Richard L./McDonald, James/Zapata, Carlos A.
Purpose: To determine the influence of nicotine upon the release of interleukin-6 (IL-6) from human periodontal ligament fibroblasts (HPLF).
Methods and Materials: The HPLF were exposed to nicotine concentrations of 125 to 1500 µg/ml for 24 hours (h). IL-6 release and cell growth were determined after exposure to the different nicotine concentrations after 24 hours. IL-6 levels were measured by enzyme-linked immunosorbent assay (ELISA). Cell counts were determined with a Coulter counter.
Results: HPLF cell counts were significantly higher than untreated cells after nicotine exposure of 375 µg/ml and significantly lower than untreated cells for cells treated with 1500 µg/ml nicotine. After 24 h of cell exposure to nicotine concentrations of 250 and 375 µg/ml, HPLF release of IL-6 per cell was significantly higher than that of untreated HPLF. HPLF treated with 1500 µg/ml, nicotine released significantly less IL-6 per cell than did untreated cells.
Conclusions: These results indicate that nicotine can increase IL-6 release from HPLF.

Keywords: nicotine, fibroblasts and Interleukin-6