Oral Biosci Med 2 (2005), No. 1 (04.07.2005)
The Incidence of Gs α Mutation in Fibro-Osseous Lesions of the Jaws using a PCR-SSCP Method
Eslami, Behnam/Kazemi, Bahram/Aghabeik Lavasani, Sanaz/Rahimi, Hessam/Yavari, Amir Saeed
Purpose: Fibro-osseous lesions of the jaws are benign lesions that replace bone, generally during childhood or adolescence. Mutation of α subunit of signal-transducing G proteins (Gsα) has been implicated in fibrous dysplasia and the Albright-McCune syndrome. In this study, we examined the Gsα mutation in 24 fibro-osseous jaw lesions.
Materials and Methods: The formalin-fixed paraffin embedded samples consisted of eight cases of fibrous dysplasia, 10 cases of cemento-ossifying fibroma, and six cases of focal cemento-osseous dysplasia were selected. Five-micrometer sections of the blocks were prepared and the DNA was then extracted. Subsequently, polymerase chain reaction (nested - PCR) and single-strand conformational polymorphism (SSCP) were carried out to detect the mutations.
Results: This mutation was detected in six fibrous dysplasia, nine cemento-ossifying fibroma and four focal cemento-osseous dysplasia samples studied. Statistical analysis of the data (chi-square test) showed no significant difference between these lesions (p=0.0506).
Conclusions: This research showed that a unique mechanism must be responsible for the creation of fibro-osseous lesions, and the possibility of other unknown mutations may be responsible for the differences of these lesions. To confirm our evaluations, DNA sequencing of the fragments is being done.
Keywords: Gsα mutation, fibro-osseous lesions, fibrous dysplasia, cemento-ossifying fibroma, focal cemento-osseous dysplasia, PCR-SSCP