Oral Biosci Med 1 (2004), No. 3 (30.09.2004)
Differential Immunohistochemical Staining in Polymorphous Low Grade Adenocarcinoma and Adenoid Cystic Carcinoma: Bcl-2, MIB-1 and TP53
Darling, Mark R./Schneider, Johann W./Muller, Christo J.F.
Overlapping clinicopathological and immunopathological features of polymorphous low-grade adenocarcinoma (PLGA) and adenoid cystic carcinoma (ACC) may present a diagnostic problem, particularly with respect to small tissue biopsies from intraoral lesions. The aim of this study was to compare expression of Bcl-2, MIB-1 and TP53 in PLGA and ACC.
Ten PLGA, 11 ACC and 19 normal salivary gland controls were stained with antibodies Bcl-2, MIB-1and TP53. Bcl-2 and TP53 immunostaining was subjectively assessed using semi-quantitative methods previously described in the literature, while a novel objective approach using a digital imaging system was used to assess MIB-1 staining.
There was increased expression of TP53, Bcl-2 and Ki-67 (MIB-1) in ACC, although the differences were ificant. However, MIB-1 was significantly increased in the solid pattern of ACC.
MIB-1 staining may be of value in discriminating between more and less aggressive tumours. Further study of larger series is needed to elucidate differences between PLGA and ACC.
Keywords: polymorphous low-grade adenocarcinoma, adenoid cystic carcinoma, MIB-1, Bcl-2, TP53